LOCUS       pDONR™/Zeo
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FEATURES             Location/Qualifiers
     misc_feature    complement(268..295)
                     /note="rrnB T2 transcription termination sequence (c)"
     misc_feature    complement(427..470)
                     /note="rrnB T1 transcription termination sequence (c)"
     primer_bind     537..552
                     /note="M13 Forward (-20) priming site"
     misc_recomb     570..668
                     /label=attL1
                     /note="attL1"
     misc_recomb     complement(1700..1796)
                     /label=attL2
                     /note="attL2 (c)"
     misc_signal     complement(1811..1830)
                     /note="T7 Promoter/priming site (c)"
     primer_bind     1838..1854
                     /note="M13 Reverse priming site"
     gene            1967..2776
                     /note="Kanamycin resistance gene"
     rep_origin      2897..3570
                     /note="pUC origin"
     vector          join(1708..3573,1..651)
                     /source="pDONR%99221"
                     /type="Donor Vector"
     misc_feature    669..691
                     /note="TEV site"
     source          692..1693
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="MGC:120703 IMAGE:7939513"
                     /tissue_type="Brain, cerebellum, PCR rescued clones"
                     /clone_lib="NIH_MGC_311"
                     /note="Vector: pCR4-TOPO with reversed insert; Clone
                     identification sequence tag: GGCCATCC"
     gene            692..1693
                     /gene="UST"
                     /gene_synonym=2OST
                     /db_xref="GeneID:10090"
                     /db_xref="HGNC:17223"
     misc_feature    692..1693
                     /gene="UST"
                     /gene_synonym=2OST
                     /note="; This cDNA sequence aligns to the human reference
                     genome (build 36) without differences"
     CDS             692..1693
                     /dnas_title="uronyl-2-sulfotransferase"
                     /gene="UST"
                     /gene_synonym=2OST
                     /codon_start=1
                     /product="uronyl-2-sulfotransferase"
                     /protein_id="AAH93668.1"
                     /db_xref="GI:62739433"
                     /db_xref="GeneID:10090"
                     /db_xref="HGNC:17223"
                     /translation="MKKKQQHPGGGADPWPHGAPMGGAPPGLGSWKRRVPLLPFLRFS
                     LRDYGFCMATLLVFCLGSLLYQLSGGPPRFLLDLRQYLGNSTYLDDHGPPPSKVLPFP
                     SQVVYNRVGKCGSRTVVLLLRILSEKHGFNLVTSDIHNKTRLTKNEQMELIKNISTAE
                     QPYLFTRHVHFLNFSRFGGDQPVYINIIRDPVNRFLSNYFFRRFGDWRGEQNHMIRTP
                     SMRQEERYLDINECILENYPECSNPRLFYIIPYFCGQHPRCREPGEWALERAKLNVNE
                     NFLLVGILEELEDVLLLLERFLPHYFKGVLSIYKDPEHRKLGNMTVTVKKTVPSPEAV
                     QILYQRMRYEYEFYHYVKEQFHLLKRKFGLKSHVSKPPLRPHFFIPTPLETEEPIDDE
                     EQDDEKWLEDIYKR"
     misc_feature    692..1693
                     /note="UST coding region"
ORIGIN      
          1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
         61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
        121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
        181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
        241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
        301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
        361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
        421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
        481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
        541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC
        601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA
        661 AGCAGGCTct gaaaacttgt actttcaagg ccgcttcctg ctcgacctgc ggcagtactt
        721 gggaaattcc acttacttgg atgaccatgg accacctcct agtaaggtac tacctttccc
        781 aagccaggtg gtgtacaaca gggtaggcaa gtgtgggagc cgtactgtgg tcttgcttct
        841 gagaatcttg tcggagaagc acggatttaa tttggtcaca tcagacattc acaacaaaac
        901 caggcttact aaaaatgaac aaatggaact gattaaaaat ataagtactg ccgaacaacc
        961 ctatttattc actcgacatg ttcatttcct caacttctca aggtttggag gagaccagcc
       1021 tgtctacatc aacatcatta gagaccccgt caaccggttc ttatccaact attttttccg
       1081 tcgctttgga gactggagag gggaacaaaa tcacatgatc cgcaccccca gcatgaggca
       1141 ggaggagcgc tacctggata tcaatgagtg tattcttgaa aactatcccg agtgctccaa
       1201 ccccaggtta ttttacatca ttccgtactt ttgtggacag catcccagat gcagggagcc
       1261 tggtgaatgg gcccttgaga gagcaaagct gaacgtgaat gaaaacttcc tgctcgtggg
       1321 gattcttgaa gagttggaag atgtgctgct gttactggaa agatttttac ctcattactt
       1381 caagggcgtg ctcagtatct acaaagaccc agagcacagg aagcttggaa acatgactgt
       1441 gacggtgaag aagactgtcc cctctcctga ggctgtgcag atcctctacc agcggatgag
       1501 atacgagtac gagttttacc actacgtcaa agagcagttc cacctgctga agcgcaagtt
       1561 tggacttaag tctcacgtca gcaagccccc cctgaggcca cacttcttta tcccaactcc
       1621 actggaaacc gaggagccaa tcgacgatga agaacaggat gatgaaaagt ggctggaaga
       1681 tatttataag aggTAGGACC CAGCTTTCTT GTACAAAGTT GGCATTATAA GAAAGCATTG
       1741 CTTATCAATT TGTTGCAACG AACAGGTCAC TATCAGTCAA AATAAAATCA TTATTTGCCA
       1801 TCCAGCTGAT ATCCCCTATA GTGAGTCGTA TTACATGGTC ATAGCTGTTT CCTGGCAGCT
       1861 CTGGCCCGTG TCTCAAAATC TCTGATGTTA CATTGCACAA GATAAAATAA TATCATCATG
       1921 AACAATAAAA CTGTCTGCTT ACATAAACAG TAATACAAGG GGTGTTATGA GCCATATTCA
       1981 ACGGGAAACG TCGAGGCCGC GATTAAATTC CAACATGGAT GCTGATTTAT ATGGGTATAA
       2041 ATGGGCTCGC GATAATGTCG GGCAATCAGG TGCGACAATC TATCGCTTGT ATGGGAAGCC
       2101 CGATGCGCCA GAGTTGTTTC TGAAACATGG CAAAGGTAGC GTTGCCAATG ATGTTACAGA
       2161 TGAGATGGTC AGACTAAACT GGCTGACGGA ATTTATGCCT CTTCCGACCA TCAAGCATTT
       2221 TATCCGTACT CCTGATGATG CATGGTTACT CACCACTGCG ATCCCCGGAA AAACAGCATT
       2281 CCAGGTATTA GAAGAATATC CTGATTCAGG TGAAAATATT GTTGATGCGC TGGCAGTGTT
       2341 CCTGCGCCGG TTGCATTCGA TTCCTGTTTG TAATTGTCCT TTTAACAGCG ATCGCGTATT
       2401 TCGTCTCGCT CAGGCGCAAT CACGAATGAA TAACGGTTTG GTTGATGCGA GTGATTTTGA
       2461 TGACGAGCGT AATGGCTGGC CTGTTGAACA AGTCTGGAAA GAAATGCATA AACTTTTGCC
       2521 ATTCTCACCG GATTCAGTCG TCACTCATGG TGATTTCTCA CTTGATAACC TTATTTTTGA
       2581 CGAGGGGAAA TTAATAGGTT GTATTGATGT TGGACGAGTC GGAATCGCAG ACCGATACCA
       2641 GGATCTTGCC ATCCTATGGA ACTGCCTCGG TGAGTTTTCT CCTTCATTAC AGAAACGGCT
       2701 TTTTCAAAAA TATGGTATTG ATAATCCTGA TATGAATAAA TTGCAGTTTC ATTTGATGCT
       2761 CGATGAGTTT TTCTAATCAG AATTGGTTAA TTGGTTGTAA CACTGGCAGA GCATTACGCT
       2821 GACTTGACGG GACGGCGCAA GCTCATGACC AAAATCCCTT AACGTGAGTT ACGCGTCGTT
       2881 CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT
       2941 GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC
       3001 GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC
       3061 AAATACTGTT CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC
       3121 GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC
       3181 GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG
       3241 AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA
       3301 CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA
       3361 TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC
       3421 CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG
       3481 ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT
       3541 CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTT
//